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We denote this end of the trajectory as the most like. The -3, -4, and -5 subclusters were at the other end of the pseudotime trajectory, exhibiting the highest expression of MAFA and PDX1 , suggesting that these subpopulations were the most mature cell subpopulations

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Fermentations were conducted in a 2.5 L bioreactor containing 1 L of YCK50D medium and a fixed temperature of 20 C