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A dose-dependent titration of human serum (0.005%2%) with BCC showed that peak luminescence was delayed with higher serum concentrations, and a linear response was observed between 0.005% and 0.1% serum (R =0.9906) before saturation (Figures S4)

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Foundational Characterisation The synthesis and initial characterisation of Melanotan 1 by Hadley and Dorr at the University of Arizona established it as a potent, stable MC1R agonist with substantially enhanced in vivo bioactivity compared to native -MSH, and confirmed its capacity to stimulate melanogenesis in pre-clinical models