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Genetic instability caused by persistent cancer cell oxidative stress will therefore lead to the increase of the malignant potential of the tumour, due to the inactivation of additional tumour suppressor genes in tumour cells, or via increasing expression of proto-oncogenes (252, 253)
Evolving procaspase-1-cleaving proteases with reduced off-target cleavage activity Although X(PC) demonstrated high selectivity for procaspase-1 over VAMP1, its transfection into mammalian cells lacking procaspase-1 (HEK293T cells) still resulted in reduced cell viability compared to a catalytically inactive X(PC) variant, consistent with residual off-target activity (Extended Data Fig
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