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Sedation or anesthesia is not included in CPT 62321 and may be billed separately if medically necessary and properly documented, depending on payer guidelines

Epidemiol Psychiatr Sci 2-17

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Phillips and Shephard, 2020)

They were permeabilized with 0.1% Triton X-100 for 15 min and then incubated with 25 ng/mL RNase for 1 h at 37 C to remove unwanted RNA contamination and enhance the specificity of the staining