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At specified time intervals (0 h, 1 h, 2 h, 4 h, 6 h, 8 h, 12 h, 24 h, 48 h), the sample solution (1.5 mL) was withdrawn from the dialysate, after which an equal volume of fresh corresponding solution (1.5 mL) was promptly added back into the dialysate to maintain the overall volume

The treated supernatant was collected in a microplate reader to detect the OD value of absorbance in each tube with an optical diameter of 0.5 cm and wavelength of 520 nm