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They found that the activity of GPx from Candida intermedia yeasts cultured in medium supplemented with 50 mg Se 4+ /L was on a level of 3.02 mU/mg protein

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Cells were imaged in fresh FluoroBrite DMEM supplemented with 4 mM glutamine, 1 mM sodium pyruvate, 10% FBS and penicillin (100 U ml 1 )/streptomycin (100 g ml 1 )/fungizone (0.25 g ml 1 )