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After sample application, the column was eluted with a linear gradient from 0 to 40% ACN in 0.1% trifluoroacetic acid at a flow rate of 25 mL/min for 30 min, and wavelength 215 nm of the effluent was monitored

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This applies even if the solution still looks clear and the vial has been stored perfectly

As more specific ETC-targeted drugs enter clinical pipelines, these can also be deployed in defined cohorts of patients with CRC to examine combination efficacy