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Next, 900 l serum extract was dried in a vacuum evaporator (Thermo), reconstituted in 90 l of 50% acetonitrile (A955, Fisher Scientific) and transferred to glass vials for LCMS analysis

After incubation with trypsin, the peptide concentration in samples was estimated using a Micro BCA Protein Assay (Thermo Scientific, #23235), and a volume corresponding to 25 g peptides was transferred to a new low-bind Eppendorf tube per sample

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