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In the gastric mucosa, four peptides (FINWLIQTKITD with Glu-C and EFIAWLVK, DFPEEVAIAEELGR, DFINWLIQTK with trypsin) belonging to GLP-1 and GLP-2 sequences were identified
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The unreacted substrates acted to reduce the Cu(II)neocuproine complex into the strongly coloured Cu(I)neocuproine complex that could be measured spectrophotometrically at 450 nm
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