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Results A counter-selection system was engineered in Saccharomyces cerevisiae based on the known feedback inhibition of gamma-glutamylcysteine synthetase ( GSH1 ) gene expression, which is rate limiting for GSH synthesis: the GSH1 ORF and the counter-selectable marker GAP1 were expressed under control of the TEF1 and GSH-regulated GSH1 promoters, respectively
Five milligrams of each therefore corresponds to approximately 3.5 mol of BPC-157 against approximately 1.0 mol of TB-500, a molar ratio near 3.5 to 1 rather than 1 to 1, and any calculation expressed in molar terms has to account for that
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