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To evaluate the short-term temporal dynamics of methylation and demethylation effects, we measured the expressions of epigenetic modifying enzyme genes
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The 3-methylglutaconyl-CoA hydratase enzyme possesses both RNA-binding and hydratase activities accounting for the official name of the gene encoding this enzyme, AU RNA-binding methylglutaconyl-CoA hydratase (AUH)
The enzyme was further purified by ion-exchange chromatography by Sepharose 4B column, eluted with NaCl gradients (Fig
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