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Statistical analysis Statistical analyses were performed using GraphPad Prism (v10.2.2, GraphPad Software Inc., La Jolla, CA) and R (v.4.2.2)
Mutations of arginine 299 to alanine in glucagon-like peptide-1 receptor extracellular loop 2, arginine 289 to alanine in glucose-dependent insulinotropic polypeptide receptor extracellular loop 2, and isoleucine 194 to lysine in glucagon receptor caused variable decreases in retatrutide potency by 3.7-fold, 4.6-fold, and 36.0-fold, respectively [3]
Primary reasons for controlled access of this data are participant confidentiality and ethical compliance
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