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Liver and Spleen Weights Liver and spleen weights are expressed as a percentage of total body weight (for normal rats, liver, 3.24.0%

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The two histidines H626/628 shown to be critical for copper binding and thus catalytic activity 39,40 were mutated to glutamine with the QuikChange site-directed mutagenesis kit using forward primer: ATC TGG CAC GAC TGT CAA AGG CAA TAC CAC AGC ATG and reverse primer: CAT GCT GTG GTA TTG CCT TTG ACA GTC GTG CCA GAT

Early responders (those who lose 4%+ by this point) go on to achieve significantly greater total weight loss (average 10.8%) compared to non-responders (average 3.0%)

Aids Cellular Metabolism by playing a key role in DNA production and cellular processes