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For the aortic samples from aged mice, first, the aortas from different treatment groups were excised and other tissues were removed, leaving only the aortic endothelium

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Thus, while e-cigarettes eliminate many combustion-related toxins present in conventional cigarettes, they still emit a complex array of hazardous chemicals, including nicotine, volatile organic compounds, nitrosamines, polycyclic aromatic hydrocarbons (PAHs), aldehydes, and heavy metals [123]

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