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This change is what can cause blurred vision

Deleting Rap1 in forebrain neurons, or specifically in SF1 neurons in that region, meant low-dose metformin no longer improved glycemia

Glutathione helps stop this damage

Ganglia were digested with collagenase A (1.5 mg ml 1 ) and trypsin (0.20 mg ml 1 ) for 1 h at 37 C and mechanically dissociated with fire-polished Pasteur pipettes