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Acid keeps the protein away from the pH at which its net charge is lowest and aggregation is most favorable, but it also means the concentrated stock is not at physiological pH and that transferring it into a buffered medium involves a pH transition where local precipitation can occur if it is done abruptly into a small volume
Blood 104:928a, 2004
PMID: 15455129
It means that every 0.1ml on your insulin syringe will contain 100mcg of the peptide, making dose calculations straightforward
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