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b-d , HCC-1806 cells were grown in control media or cystine-free (n = 8 technical replicates from 2 independent experiments
provides glutathione IV drip classes under the supervision of certified medical personnel at all times
Tuy nhin, v t v khng t thc hin c ti nh nn mt s ngi cn e ngi s dng
Following 3 h of drug treatments, 5 M of the cell-permeable, ROS-sensitive dye CellROX Deep Red (Invitrogen, Stockholm, Sweden) was added to each sample and incubated at 37 C for 30 min
Real-time qPCR was performed using an Applied Biosystems QuantStudio 12 K Flex real time PCR system (Thermo Fisher Scientific, San Jose, CA, USA) with THUNDERBIRD SYBR qPCR Mix (Toyobo), 6 pmol of primers, and aliquots of cDNA
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