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Then, the color-pulsed Doppler approach was used to assess uterine blood flow by measuring the Doppler indices [resistive index (RI), pulsatility index (PI)
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The next day, the mixtures were subjected to centrifugation at 12,000 rpm for 15 min, and the resultant pellets were resuspended in PBS buffer, followed by centrifugation at 12,000 rpm for 15 min to remove insoluble fractions
The collagen synthesis stimulation represents GHK-Cu's most studied effect, with research showing increased fibroblast activity, enhanced collagen type I and III production, and improved dermal matrix organization during treatment periods
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