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We therefore generated new cohorts of mice lacking Gipr in the hypothalamus (Gipr hypo-KO ), by targeted injection of AAV-Cre or control AAVGFP (Gipr hypo-GFP ) into the paraventricular nucleus (PVN) (Fig

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04 Inject down the wall Insert the needle into the peptide vial and slowly release the BAC water down the inside wall, not directly onto the powder