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However, the pathophysiology-based disease mechanism of MDD is not yet fully understood, and diagnosis is also conducted through interviews with clinicians and patients

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Biochemical analysis Serum hormonal assays Serum samples were subjected to hormonal tests for estradiol (E2), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) utilizing chemiluminescent detection techniques using magnetic particle division (Beckman Coulter-Access 2 immunoassay system, Irving, TX, USA)

1 The incidence of antibody formation has been shown to decrease with time (over 3 years)