For Caspase-3 staining, the tissues were blocked with 10% normal horse serum for 30 min then incubated with Caspase-3 antibody (1:300, BD Biosciences, Macquarie Park, NSW, Australia) overnight, followed by secondary antibodies (Goat anti-rabbit HRP, 1:200, Vector Laboratories, Burlingame, CA, USA) for 45 min
As always, consult with a healthcare professional for personalized guidance and support tailored to individual needs and circumstances
Outcome Measurements All primary (behavioral measurements) and secondary outcomes (intracellular glutathione levels and adverse events) were obtained at baseline and study end
For testing on a first-time melanotan II subject, it is important to start with the lowest dose possible, before increasing as needed to achieve the desired outcome
Reply Following