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In any case, we have yet to find any robust differences in nascent ER protein trafficking, processing, or aggregation in cells in which TCA activity is manipulated other than apparently enhanced clearance of NHK (Figure 4D)
In parallel, a negative control was performed by incubating cells with bacteria for 10 min on ice
[5] Pyridoxine (vitamin B) is converted to pyridoxal-5-phosphate, a coenzyme for over 100 enzymes in amino acid metabolism
Our team has worked with research groups comparing metabolic outcomes across both routes, and the confounding variable is almost always adherence
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