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Immune cells generally express low GLP-1R, yet macrophages and T lymphocytes carry functional levels, suggesting an immunomodulatory axis [28,29,30]
Samples were permeabilized, blocked (Dako, #X0909) and then incubated with rabbit anti-glucagon (Santa Cruz, 1:200 discontinued) and mouse anti-GLP-1 (736) amide (Abcam #ab26278, 1:200) antibodies
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The amphiphilic compound N-acetil--D-glucosaminil-PEG900-docosanato (C22PEG900GlcNAc 1509 g/mol) was synthesized by esterification of N3-PEG900-OH with docosanoic acid, followed by a click chemistry reaction with a modified carbohydrate, as described in the literature
