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Tissue preparation Rats were anaesthetized with an intraperitoneal injection of ketamine (100 mg/kg) and dexmedetomidine (20 mg/kg) in a volume of 10 ml/kg and were then perfused transcardially with 300 ml phosphate buffered saline (PBS) and thereafter with 300 ml 4% paraformaldehyde (PFA, Sigma-Aldrich) in a 0.1 M phosphate buffer (PB, pH 7.4)