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One individual per group was euthanized before addressing the next animal in each group to render differences arising from the time of day at euthanization (e.g., cecal mass changes resulting from differences in time post-food consumption) comparable across all groups

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NHS advice says B12 is not naturally found in foods such as fruit, vegetables and grains, so vegans may not get enough without fortified foods or supplementation

The NIH Office of Dietary Supplements, citing Institute of Medicine recommendations, advises that it is advisable for those older than 50 years to meet their RDA mainly by consuming foods fortified with vitamin B12 or a supplement containing vitamin B12. This recommendation reflects the fact that 10 to 30% of older adults lose the ability to efficiently absorb naturally occurring B12 from food

Formulation additives Many compounded tirzepatide formulations include additional ingredients like vitamin B12, glycine , vitamin B6 , or niacinamide