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Hepatic stellate cells (HSCs) are central drivers of liver fibrosis and cancer progression, differentiating into myofibroblasts and cancer-associated fibroblasts

Several technical reasons may explain our distinctive findings, including differences in the microbiome sampling site, the limited n per group, singly housing mice, or the use of highly similar lean, Western, and high-fat diets which systematically exchanged fat content with carbohydrate content

The tolerable upper limit has not been established