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Immunostaining was performed by seeding cells on glass coverslips, fixing with paraformaldehyde, permeabilizing with Triton X-100, and then incubating with primary anti-NF-B p65 antibody, followed by Alexa Fluor 546-conjugated secondary antibody

We found that, regardless of the total dose of rHuEPO used, this substance improves the main hematological (total hemoglobin mass, hemoglobin concentration, and hematocrit) and physiological (maximal oxygen uptake and peak oxygen uptake) parameters, while the maximal performance-related parameters (mainly, maximal power output, and peak power output) also tend to increase

Frequency: Once to twice per week, depending on activity level and therapeutic intent

Consent for publication Written informed consent for the publication of this case and all of the accompanying images were obtained from the patient and his guardian

Remember to rest, eat a balanced diet, and avoid common mistakes such as alcohol consumption and smoking