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Immunohistochemistry The slices were incubated in the blocking buffer (10% horse serum, 0.1% Triton X-100, 0.02% sodium azide in PBS) with the primary antibody at room temperature overnight

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The Trial The ATTAIN-MAINTAIN trial enrolled patients who had achieved substantial weight loss that had plateaued while taking weekly injectable GLP-1s in the SURMOUNT-5 trial

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The first week, adults get 1000 mcg daily