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A 2025 systematic review found no clinical safety data and only one clinical study, so animal findings do not establish human efficacy
In humans, TB4 is present in high concentrations in all tissue types except red blood cells, with highest levels occurring in platelets, white blood cells, plasma, and wound fluid.17 TB4 is an important actin-binding protein that helps actin perform various functions, such as muscular contraction and maintaining proper cell shape, movement, and division.18 19 These effects on cells are why some experts suggest supplemental TB4 can improve the repair and recovery of muscle and other soft tissues,20 21 promote wound healing,22 reduce inflammation, and more.23 Researchers also note that TB4 is critical for the repair and regeneration of eye, heart, skin, nerve, and brain tissue after injury.4 The derivative TB-500 is based on one of the active sites of TB4, specifically the peptide segment responsible for the compounds actin-binding and cell migration abilities.4 Since most of TB4s potential benefits are attributed to its actin-binding properties, it can be assumed that the same applies to TB-500
(2020) 31:11530.e6
doi:10.1073/pnas.0900206106
