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Homogenates were centrifuged at 800 g for 10 min at 4 C, and the supernatant was sonicated on ice for 20 s before protein quantification (BCA protein assay, Pierce, Rockford, IL, USA)

The result was nonsignificant among all groups euthyroid, hypothyroidism control, IF + Vit

This distinction matters enormously for long-term outcomes

STORAGE CONDITIONS: Store in a cool, dry place at room temperature, away from direct sunlight