This underscores that mimicking a more physiological environment enables a more accurate representation of pathogenic potential in in vitro infection models
With sufficient glutathione present, NAPQI is very quickly metabolized into sulfate and cysteine compounds, which are readily excreted through the kidneys.[ref] However, when it isnt neutralized, NAPQI is highly reactive and can cause oxidative stress and damage to the liver cells, hepatocytes, leading to cell death
I do not use other forms of reduced glutathione, as they have poor absorption and do not generally make it through the digestive system intact
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This is how drug release kinetics can be altered depending on their solubility and the pH inside and outside the membrane