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At the end of the incubation period cells were lysed by addition of PathHunter detection reagents and luminescent signal was subsequently recorded from each well

Best practices: Finish dinner at least 2-3 hours before bed Keep dinner moderate in size (not your biggest meal) If you need an evening snack, choose something light and protein-rich (Greek yogurt, cottage cheese) Avoid high-fat evening meals that slow digestion further Learn more about optimizing meal timing in our guide on building a balanced meal routine

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