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Lentiviral production and generation of stable cell lines Guide RNAs targeting SLC2A1 (GLUT1 coding gene) or DHHCs were designed using Cas9 target design online tool ( Three different promising guide RNAs were chosen and cloned into the lentiCRISPRv2 lentiviral vector with a selectable marker of puromycin

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1b), the enzyme has no catalytic function