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Abbreviation definitions: BSO (buthionine sulfoximine, a known inhibitor of glutathione synthesis), WPI (whey protein isolate), and NAC (N-acetyl cysteine)

Ethics statement The animal study was approved by the Institutional Animal Care and Use Committee at Cairo University, Cairo, Egypt (Vet CU13102024983)

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Protocol Overview At 5 mg/mL a single 10 mg vial holds 2 mL of usable solution twenty 0.5 mg doses, or roughly two to three weeks of a 1 mg daily loading phase from one vial

So increasing hormones are gut peptides that are secreted after nutrient intake, they stimulate the increase in insulin secretion by two to threefold