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(2) To remove hydrophobic substances, wash the column with 3-4 bed volumes of 70% ethanol or 2 bed volumes of 1% Triton X-100 followed by 5 bed volumes of PBS (pH 7.4) washed

Research has shown that IL-6 and chemokine CC motif ligand 2 (CCL2) levels increase in human umbilical vein endothelial cells (HUVECs) when the radiation dose is as low as 0.5 Gy, if the radiation dose increases to 2 Gy, levels of IL-8, TGF-, TNF-, IL-1, IFN-, and adhesion molecules such as ICAM-1, VCAM-1, E-selectin and VEGF increase significantly (Baselet et al