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The study is published Jan
1, 2 IGF-1R is primarily activated by its high-affinity ligands, IGF-1 and IGF-2, though it can also bind insulin with lower affinity
The supernatant was analysed by LC-MS
After 24 h, cells were rinsed with PBS and incubated with 10% trichloroacetic acid (TCA) at 4 C for 30 min to precipitate the proteins
It works by triggering the release of certain neurotransmitters in the brain that can decrease appetite
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