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Finally, apoptosis analysis was performed using a flow cytometer (CytoFlex SRT, Beckman Coulter)
ANTIOXIDANTS Antioxidants are the substances which neutralize the harmful affects of free radicals and protect the cell against the oxidative stress They are Superoxide dismutase present in cytoplasm and produced by mitochondria
Moreover, some studies have shown that the ChaC1 protein is significantly upregulated in various adverse conditions, such as diabetes, viral infection and atherosclerosis, providing cells with the amino acid nutrients they need under stressful situations but reducing GSH levels
However, a functional assignment of these residues remains difficult because of the absence of structures with transition-state analogues that allow the discrimination between the glutathione moieties from the GSSR substrate or GSH (Fig