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That is why the entire schedule below is expressed as a frequency curve daily, then five times weekly, then two-to-three times weekly rather than as a dose curve

Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

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One of the well-known senolytic drugs, Navitoclax, also holds the potential for use in One-Two Punch therapy [138]

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