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For example, a GLP-1/oestradiol conjugate reduced food intake and body weight in different rodent models of the metabolic syndrome, displaying greater efficacy than GLP-1 alone [100]

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Fluorescence emission readings were analyzed using Rotor-Gene Q software (Qiagen, Germany)

Wakefulness time measurement To evaluate the impact of Pichia pastoris secreted recombinant peptides on insomnia, we analyzed the increase in wakefulness time among different groups of mice: Control, Model, GABA, DSIP-CBBBP, DSIP, and CBBBP