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GSH is found in all mammalian tissues as the most abundant non-protein thiol that defends against oxidative stress and possess a distinctive stability provided by a -carboxyl bond within the molecule (Figure 1A)
Probably due to the binding preference of APAP to the active site of each P450 isomer, it is oxidized via two pathways to form the toxic intermediate N-acetyl-p-benzoquinone imine (NAPQI) and the non-toxic catechol metabolite 3-hydroxy-APAP (3-OH-APAP) (Chen et al., 1998)
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