Age is like a little demon that is slowly robbing you of your tissue
After the assessment, each bull ejaculate ( n = 9) with semen volume 4 ml, sperm cell concentration 1.0 10 9 /ml, progressive motility 75%, and sperm viability 80% were split into aliquots according to the experimental design and applied for the following experiments ( Optimization of SeNPs concentration for semen cryopreservation The basic semen extender used in this study was a Tris-based extender as distilled water supplemented with 28 mM fructose, 104 mM citric acid, 297 mM Tris buffer, 5.0% (v/v) glycerol, and 20% (v/v) egg yolk
Additives like B12, B6, NAD+, and glycine may be included to support comfort and minimize side effects
,
This is a preview of subscription content, access via your institution Access options Subscribe to this journal Receive 12 print issues and online access 261,46 per year only 21,79 per issue Buy this article Purchase on SpringerLink Instant access to the full article PDF