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A standard expression induction protocol was based on shifting log-phase cultures (A600 = ~0.6) from 37 C and adding IPTG (1 mM)

By this mechanism, acetaldehyde is converted into a harmless conjugate

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In a large cohort study from the United States involving more than 18,000 overweight or obese individuals, Rodriguez et al reported that the average weight loss after one year was 8.3% with semaglutide and 15.3% with tirzepatide

doi: 10.3390/ijms19103206 166 KuglerA.StensjK