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Going forward, several key considerations should be addressed to further expand our understanding and optimize the use of GLP-1 RAs and SGLT2is in PCOS: Longer-term studies: The current trials have relatively short durations, necessitating the need for longer-term studies to assess the sustained efficacy and safety of these interventions in PCOS

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For tumour xenograft models, 1 10 6 HCC-1806 cells or 5 10 6 PC3 cells were injected into the right fourth mammary fat pad or hind flank of athymic nude NU/J mice (The Jackson Laboratory, 002019) in 100 l sterile PBS or a 1:1 mixture of cell suspension in PBS and Matrigel (Corning, 356237) using a 29-gauge 1 ml insulin syringe

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GLP-1RA users and non-users were matched using propensity scores