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After 24 h of co-culture with G/D-CuP, NIH/3T3 cells displayed well-spread morphology and high viability, as demonstrated by both 3D and 2D Calcein-AM fluorescence imaging via CLSM (Supplementary Fig
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The plume angle (i.e., the half-angle at the spray cone vertex) and the intranasal nozzle insertion depth were selected ( 2.3 On how to hold the spray bottle A key parameter for targeted delivery is the direction of the nasal spray axis, as the sprayed droplet trajectories are often inertia-dominated (Finlay, 2001
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