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W., Binns, S
Perfused upper small intestine Male mice ( n = 6) were anaesthetised as described above, and the upper small intestine was isolated and perfused as previously described [23]
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Prior to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), 20 g of frozen, ground, and fresh tissue was homogenized in 20 mL of extraction buffer containing 100 mmol L 1 TrisHCl (pH 6.8), 10 g L 1 SDS, 10 mmol L 1 dithiothreitol (DTT), 1 mmol L 1 ethylenediaminetetraacetic acid disodium salt (EDTA-2Na), and a protease inhibitor mixture (cat
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