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The acid helps leach nutrients out of the bones and into the broth, essentially liquifying the bone marrow so that by the end of the cooking process the bones nearly crumble in your hands

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Both 25-hydroxylases (CYP2R1 and CYP27A1) were expressed in the liver, while CYP27A1 expression was seen only in cultured hepatocytes (FL83B mouse hepatocyte cell line)