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After chondrocytes reached 8090% confluency, the cells were dissociated with Trypsin/EDTA (Gibco/Thermo Fisher Scientific) and passaged

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To ensure energy balance, the brain must balance energy expenditure and conservation in coordination with internal metabolic statesdynamic regulation of motivational cognition may represent a higher-order mechanisms by which energy-expending behaviour is aligned with energy allostatic goals

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