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The optical density was subsequently measured at a wavelength of 450 nm using a multi-function microplate reader (Molecular Devices, United States)
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Establishment of K7M2-Luc orthotopic osteosarcoma mouse model and therapeutic effect evaluation Tumor model establishment To establish orthotopic model, K7M2 cells (3 10 6 cells/well) were dispersed in PBS buffer and implanted into right tibia of BALB/c mice
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