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Draw up of water( 5 ml Bacteriostatic Sodium Chloride 0.9% )
Cell viability after 24 h treatment was assessed using ApoTox-Glo triplex assay (Promega) according to the manufacturers protocol
Wait and Withdraw: Once the syringe is empty, wait for about 5-10 seconds before withdrawing the needle
It is responsible for cellular respiratory & protecting the very sensitive cell membranes
These results suggested that chronic hypoxia promoted the expression of Acer2 by activating HIF-2
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