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Nevertheless, we briefly evaluated the involvement of DNA damage in the MTX-induced root inhibition by phenotyping several mutants defective in response to DNA damage
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PCs ( P11) were seeded into collagen type IV and fibronectin coated dishes in Dulbeccos modified Eagles medium (DMEM, Life Technologies, Thermo Fisher Scientific, USA) supplemented with 20% FBS, 1% Glutamax (Life Technologies) and gentamicin (50 g/ml)
Diets rich in fat and sugar, combined with physical inactivity, contribute to a sustained positive energy balance
[58] Medical uses [edit] Treatment of deficiency [edit] Severe vitamin B 12 deficiency is initially corrected with daily intramuscular injections of 1000 g of the vitamin, followed by maintenance via monthly injections of the same amount or daily oral dosing of 1000 g
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